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KMID : 0829220050290020083
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Korean Journal of Oral and Maxillofacial Pathology
2005 Volume.29 No. 2 p.83 ~ p.92
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Role of Nuclear Factor Ι-C in Odontoblast Differentiation
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Bae Hyun-Sook
Jeong Moon-Jin
Kim Heung-Joong
Park Min-Ju
Ahn Seong-Min
Gronostajski
Park Ju-Cheol
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Abstract
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Nuclear factor I (NFI) was discovered as a protein required for adenovirus DNA replication in vitro, but it is now clear that NFI protein plays an important role in the expression of many cellular genes. NFI-C null mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and thus molar lacking roots while other tissues/organs in the body, including ameloblasts appear to be unaffected and normal. However, little is known about the mechanism of NFI-C function in odontoblast differentiation and dentin formation. In this study, in order to elucidate the molecular mechanisms of odntoblast differentiation, we examined morphological characteristics of the aberrant odontoblast in NFI-C null mice. we also evaluate the expression of dentin sialophosphoprotein (DSPP) and bone sialoprotein (BSP) mRNAs in the MDPC-23 cells by northern analysis after over-expression and inactivation of NFI-C into mouse MDPC-23 cells. Odontoblasts of the NFI-C null mouse were round in shape, lost their polarity, organized as a sheet of cells, and trapped in osteodentin-like mineralized tissue. Abnormal odontoblasts of NFI-C null mouse revealed the absence of an intercellular junctional complex known as the terminal webs. MDPC-23 cells started to express DSPP mRNA beginning from the postnatal day of 14 and showed a steady increase as differentiating into odontoblasts. Over-expression of NFI-C increased the expression of DSPP mRNA. Inactivation of NFI-C induced BSP mRNA expression. These results suggest that NFI-C plays an important role in odontoblast differentiation in a cell-type specific manner and thus in dentin formation.
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KEYWORD
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NFI-C, Odontoblast differentiation, Root formation, DSPP, BSP
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